91蝌蚪91九色-伊人久久影院-精品伊人-欧美福利在线观看-国户精品久久久久久久久久久不卡-色婷婷av一区二区三区之e本道-久久久久一-久久99热这里只频精品6学生-av免费试看-亚洲欧美一级-97超碰自拍-在线观看免费人成视频-亚洲欧美自拍另类-潘金莲性xxxxhd-缅甸黄色片-超碰久操-一级片高清-www.黄免费-中国精品毛片-草莓视频成人在线-国产第一页福利-秋霞午夜久久-精品久久免费观看-欧美性白人极品1819hd-丰满少妇熟乱xxxxx视频-国产一区你懂的-午夜网站视频-人成午夜视频-国产亚洲黄色片-国产男男gay做受ⅹxx高潮

【文獻標題】Dietary prebiotic inulin benefits on growth performance, antioxidant capacity, immune response and intestinal microbiota in Pacific white shrimp (Litopenaeus vannamei) at low salinity

【作者】Li Zhou，Huifeng Li，Jian G. Qin，et.al

【作者單位】海南大學（Hainan University）

【文獻中引用產品】

蝦蛋白酶（Protease）ELSIA試劑盒

蝦淀粉酶（Amylase）ELISA試劑盒

蝦脂肪酶（Lipase）ELISA試劑盒

【關鍵詞】Low salinity，Inulin，Growth performance，Antioxidant capacity，Intestinal microbiota，Litopenaeus vannamei

【DOI】doi.org/10.1016/j.aquaculture.2019.734847

【影響因子(IF)】3.42

【出版期刊】《Aquaculture》

【產品原文引用】

2.7. Biochemical assays

The entire intestinal of three shrimps mixed as one sample, the hepatopancreas of one shrimp as one sample, each group have six samples. All samples were weighed and homogenized with 9 vol (v/w)of 0.86% physiological saline. Then, the homogenate was centrifuged at 2500 ×g at 4 °C for 10 min and the supernatant was collected for biochemical assays according to the manufacturer's instruction.Activities of intestinal protease, amylase, and lipase were measured using an enzyme-linked immune sorbent assay (ELISA) kit (Shanghai Hengyuan Biotechnology Co, Ltd) (Li et al., 2019). Phenol oxidase (PO)activity in hepatopancreas was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Nanjing Jiancheng Institute, China).The contents of malondialdehyde (MDA), total superoxide dismutase (T-SOD), catalase (CAT), acid phosphatase (ACP) in hepatopancreas were measured by using specific commercial assay kits (Nanjing Jiancheng Institute, China) (Zhang et al., 2008). Results were recorded on a microplate reader (Epoch, BioTek, USA).Total SOD activity was determined by using WST-1 method. One unit of SOD activity was defined as the amount of enzyme required for 1 mg tissue proteins in 1 mL of a reaction mixture SOD inhibition rates to 50% as monitored at 550 nm.CAT activity was determined by using ammonium molybdate colorimetric method. One unit of CAT activity was defined as 1 mg tissue proteins consumed 1 μmol H2O2 at 405 nm for 60 s. MDA content was determined by using TBA method, which was measured at 532 nm. ACP activity was determined by using colorimetric method. One unit of ACP activity was defined as 1 mg tissue proteins produced 1 mg phenol at 37 °C for 30 min as monitored at 550 nm. Activities of protease, amylase, lipase and phenol oxidase were measured using the enzyme-linked immunosorbent assay (ELISA) and the absorbance (OD) was measured at 450 nm. Total protein content was measured by using Coomassie brilliant blue method.

完整版PDF文獻請咨詢在線客服或者聯系我司業務員！

更多公司福利請關注“恒遠生物”VX公眾號！